Cells were lysed with RIPA lysis buffer (50mM Tris HCl pH 7.5, 150mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulfate, 10mM EDTA, 1% sodium deoxycholate) where each mL was supplemented with 10uL of Phosphatase Inhibitor Cocktail A, B, and C (ThermoFisher Scientific, Waltham, MA). Citiolone CDK2 inhibition tend crucial to the Rabbit Polyclonal to … Continue reading Cells were lysed with RIPA lysis buffer (50mM Tris HCl pH 7